Journal: Scientific Reports

Article Title: An organ-on-chip device with integrated charge sensors and recording microelectrodes

doi: 10.1038/s41598-023-34786-5

Figure Lengend Snippet: Biocompatibility and electrophysiological proof of concept experiments: ( a , b ) HiPSC-derived cortical neurons differentiated (7 days) and matured (14 days) on-chip. After staining, \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\beta 3$$\end{document} β 3 tubulin-positive neurons (green) and synaptic vesicles (red) are visible. ( c ) Compatibility with Multichannel Systems MEA recording setup of the PCB and the chip. ( d ) Inset showing the chip and the well with medium and cells. ( e ) Single-electrode trace signal from the MEA recordings. ( f ) The addition of Picrotoxin (50 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\upmu \hbox {M}$$\end{document} μ M ) caused an increase in fire rate.

Article Snippet: Lastly, the hiPSC-derived cortical neurons were matured and maintained in BrainPhys hiPSC Neuron kit media (05795, StemCell Technologies) for the remainder of the experiment.

Techniques: Derivative Assay, Staining